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proteome profiler human pluripotent stem cell array  (R&D Systems)


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    R&D Systems proteome profiler human pluripotent stem cell array
    Proteome Profiler Human Pluripotent Stem Cell Array, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 177 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/proteome+profiler+human+pluripotent+stem+cell+array+kit/Human+Pluripotent+Stem+Cell+Functional+Identification+Kit/pmc12408728-54-7-14
    Average 96 stars, based on 177 article reviews
    proteome profiler human pluripotent stem cell array - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Expressing:

    Article Title: An organoid model of colorectal circulating tumor cells with stem cell features, hybrid EMT state and distinctive therapy response profile
    Article Snippet: .. The expression of stem cell- cancer- and stress-related proteins was evaluated with Proteome Profiler Human Pluripotent Stem Cell Array Kit (#ARY010), Proteome Profiler Human XL Oncology Array (#ARY026) and Proteome Profiler Human Cell Stress Array Kit (#ARY018) all from R&D Systems. ..

    Article Title: Tumor-Activated Mesenchymal Stromal Cells Promote Osteosarcoma Stemness and Migratory Potential via IL-6 Secretion
    Article Snippet: .. To assess expression of stem cell-related markers in CSC from osteosarcoma that were co-cultured with MSC and in respect to parental cell line, protein extracts were obtained and quantified with Proteome Profiler Human Pluripotent Stem Cell Array Kit (R&D) lysis buffer. ..

    Article Title: The IL-6R and Bmi-1 axis controls self-renewal and chemoresistance of head and neck cancer stem cells
    Article Snippet: .. The proteome profiler human pluripotent stem cell array kit (R&D Systems) was used to evaluate expression of stem cell markers, according to the manufacturer’s instructions. .. Briefly, UM-SCC-1, UM-SCC-22A, or UM-SCC-22B cells were plated, serum-starved overnight, and treated with 0–1 μM Cisplatin (Sigma) and/or 0–0.1 μM Tocilizumab (Genentech) for 24 h. Lysates were extracted and incubated with the antibody-spotted array following the manufacturer’s instructions.

    Article Title: Towards a standardised method for the characterisation and isolation of colorectal cancer stem cells by SdFFF and UHF-DEP: highlighted by transcriptomic analysis
    Article Snippet: .. The proteome profiler human pluripotent stem cell array kit (R&D Systems) was used to evaluate the expression of cancer stem cell markers, according to the manufacturer’s instructions. .. Briefly sorted cell lysate proteins were extracted using RIPA buffer (Sigma-Aldrich) and sonication (VibraCellTM).

    Article Title: Tumor-Activated Mesenchymal Stromal Cells Promote Osteosarcoma Stemness and Migratory Potential via IL-6 Secretion.
    Article Snippet: .. Proteome profiler array To assess expression of stem cell-related markers in CSC from osteosarcoma that were co-cultured with MSC and in respect to parental cell line, protein extracts were obtained and quantified with Proteome Profiler Human Pluripotent Stem Cell Array Kit (R&D) lysis buffer. ..

    Lysis:

    Article Title: Tumor-Activated Mesenchymal Stromal Cells Promote Osteosarcoma Stemness and Migratory Potential via IL-6 Secretion
    Article Snippet: .. To assess expression of stem cell-related markers in CSC from osteosarcoma that were co-cultured with MSC and in respect to parental cell line, protein extracts were obtained and quantified with Proteome Profiler Human Pluripotent Stem Cell Array Kit (R&D) lysis buffer. ..

    Article Title: Tumor-Activated Mesenchymal Stromal Cells Promote Osteosarcoma Stemness and Migratory Potential via IL-6 Secretion.
    Article Snippet: .. Proteome profiler array To assess expression of stem cell-related markers in CSC from osteosarcoma that were co-cultured with MSC and in respect to parental cell line, protein extracts were obtained and quantified with Proteome Profiler Human Pluripotent Stem Cell Array Kit (R&D) lysis buffer. ..

    Marker:

    Article Title: Induced hepatic stem cells are suitable for human hepatocyte production
    Article Snippet: .. Stem cell-specific marker proteins were measured using the Proteome Profiler Human Pluripotent Stem Cell Array Kit (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions. .. In brief, the hiPSCs were seeded on a feeder layer of MEF cells (ReproCELL Inc., Kanagawa, Japan) and seeded at 1.5 × 10 5 cells per 10-cm plate. iPSCs were cultured in 100 mm dishes in Primate ES Cell Medium (ReproCELL Inc., Kanagawa, Japan) to ∼80% confluence.

    Article Title: Induced hepatic stem cells are suitable for human hepatocyte production.
    Article Snippet: .. Stem cell-specific marker proteins were measured using the Proteome Profiler Human Pluripotent Stem Cell Array Kit (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions. .. In brief, the hiPSCs were seeded on a feeder layer of MEF cells (ReproCELL Inc., Kanagawa, Japan) and seeded at 1.5 3 105 cells per 10-cm plate. iPSCs were cultured in 100 mm dishes in Primate ES Cell Medium (ReproCELL Inc., Kanagawa, Japan) to 80% confluence.

    other:

    Article Title: Induced hepatic stem cells are suitable for human hepatocyte production.
    Article Snippet: In brief, the hiPSCs were seeded on a feeder layer of MEF cells (ReproCELL Inc., Kanagawa, Japan) and seeded at 1.5 3 105 cells per 10-cm plate. iPSCs were cultured in 100 mm dishes in Primate ES Cell Medium (ReproCELL Inc., Kanagawa, Japan) to 80% confluence.



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    Image Search Results


    FIGURE 1 EVsCSC and EVsDCC isolation and characterization. (A) Representative images of CSCs and DCCs analyzed for tdTomato fluorescence, scale bar = 25 μm. (B) Gene expression of stemness reporters ALDH1A1, Nanog and Oct-4 detected by qPCR for MDA-MB-231 subpopulations. CSC refers to tdTomato + cells cultured in stem cell maintenance media while DCC indicates cells cultured with a minimal presence of CSC (1%-2%) to avoid de-differentiation. CNRQ stands for Calibrated Normalized Relative Quantity with respect to GAPDH and Actin housekeeping genes. (C) 2D laminin invasion assay comparing CSC and DCC cells. (D) CryoTEM imaging of isolated EVsCSC and EVsDCC. (E) Stochastic Optical Resolution Microscopy (STORM) imaging of isolated EVs previously labeled with DiD. (F) Size distribution by nanoparticle tracking analysis (NTA) of EVsCSC and EVsDCC. (G) EVs typical markers, CD81, TSG101 and ALIX and cell lysate control, β-tubulin identified by Western blot. Twenty micrograms of total protein was loaded per lane and cell extracts (CE) were included as controls. (H) ALDH1A1 and CD44 protein cargo in EVCSC and EVsDCC examined by Western blot. (I) Protein cargo of EVCSC and EVsDCC assayed with a Human Pluripotent Stem Cell Array, showing higher content of OCT4, SOX2 and E- Cadherin in EVCSC. In H and I, quantification of blots are shown as the fold change in the band/dot intensity of EVsCSC/EVsDCC. Differences are significant for ALDH1A1, CD44, SOX2 and E-Cadherin (*P < .05)

    Journal: International journal of cancer

    Article Title: Extracellular vesicles secreted by triple-negative breast cancer stem cells trigger premetastatic niche remodeling and metastatic growth in the lungs.

    doi: 10.1002/ijc.34447

    Figure Lengend Snippet: FIGURE 1 EVsCSC and EVsDCC isolation and characterization. (A) Representative images of CSCs and DCCs analyzed for tdTomato fluorescence, scale bar = 25 μm. (B) Gene expression of stemness reporters ALDH1A1, Nanog and Oct-4 detected by qPCR for MDA-MB-231 subpopulations. CSC refers to tdTomato + cells cultured in stem cell maintenance media while DCC indicates cells cultured with a minimal presence of CSC (1%-2%) to avoid de-differentiation. CNRQ stands for Calibrated Normalized Relative Quantity with respect to GAPDH and Actin housekeeping genes. (C) 2D laminin invasion assay comparing CSC and DCC cells. (D) CryoTEM imaging of isolated EVsCSC and EVsDCC. (E) Stochastic Optical Resolution Microscopy (STORM) imaging of isolated EVs previously labeled with DiD. (F) Size distribution by nanoparticle tracking analysis (NTA) of EVsCSC and EVsDCC. (G) EVs typical markers, CD81, TSG101 and ALIX and cell lysate control, β-tubulin identified by Western blot. Twenty micrograms of total protein was loaded per lane and cell extracts (CE) were included as controls. (H) ALDH1A1 and CD44 protein cargo in EVCSC and EVsDCC examined by Western blot. (I) Protein cargo of EVCSC and EVsDCC assayed with a Human Pluripotent Stem Cell Array, showing higher content of OCT4, SOX2 and E- Cadherin in EVCSC. In H and I, quantification of blots are shown as the fold change in the band/dot intensity of EVsCSC/EVsDCC. Differences are significant for ALDH1A1, CD44, SOX2 and E-Cadherin (*P < .05)

    Article Snippet: Human pluripotent stem cell antibody array (R&D Systems, ARY010) was performed in accordance with the manufacturer's instructions.

    Techniques: Isolation, Fluorescence, Gene Expression, Cell Culture, Invasion Assay, Imaging, Microscopy, Labeling, Control, Western Blot